{"product_id":"pnc-28-p53-derived-research-peptide","title":"PNC-28 Research Peptide – 20mg","description":"\u003ch4 data-path-to-node=\"13\"\u003e\u003cstrong\u003ePNC-28 Peptide (Lyophilized)\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cp data-path-to-node=\"14\"\u003e\u003cb data-path-to-node=\"14\" data-index-in-node=\"0\"\u003eSequence:\u003c\/b\u003e ETFSDLWKLL-KKWKMRRNQFWVKVQRG (p53 17-26 + Penetratin) \u003cb data-path-to-node=\"14\" data-index-in-node=\"64\"\u003eFormat:\u003c\/b\u003e Sterile Lyophilized Powder (Vial) \u003cb data-path-to-node=\"14\" data-index-in-node=\"106\"\u003eMechanism:\u003c\/b\u003e MDM2-Mediated Membranolysis (Poptosis) \u003cb data-path-to-node=\"14\" data-index-in-node=\"156\"\u003ePurity:\u003c\/b\u003e \u0026gt;98% (HPLC) \u003cb data-path-to-node=\"14\" data-index-in-node=\"176\"\u003eContent:\u003c\/b\u003e 20mg per vial\u003c\/p\u003e\n\u003chr data-path-to-node=\"15\"\u003e\n\u003ch3 data-path-to-node=\"16\"\u003e\u003cb data-path-to-node=\"16\" data-index-in-node=\"0\"\u003eProduct Overview\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp data-path-to-node=\"17\"\u003e\u003cb data-path-to-node=\"17\" data-index-in-node=\"0\"\u003ePNC-28\u003c\/b\u003e is a synthetic fusion peptide derived from the p53 tumor suppressor protein. It consists of the p53 binding domain (residues 17-26) linked to a cell-penetrating \"penetratin\" sequence.\u003c\/p\u003e\n\u003cp data-path-to-node=\"18\"\u003eThis unique design allows PNC-28 to target malignant cells that overexpress MDM2 on their plasma membrane. Unlike traditional therapies that induce apoptosis, PNC-28 triggers a rapid form of necrosis known as \u003cb data-path-to-node=\"18\" data-index-in-node=\"209\"\u003e\"poptosis\"\u003c\/b\u003e (peptide-induced transmembrane pore formation), making it a critical tool for researching chemotherapy-resistant cell lines.\u003c\/p\u003e\n\u003chr data-path-to-node=\"19\"\u003e\n\u003ch3 data-path-to-node=\"20\"\u003e\u003cb data-path-to-node=\"20\" data-index-in-node=\"0\"\u003eMechanism of Action: The Necrotic Trigger\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp data-path-to-node=\"21\"\u003ePNC-28 operates via a biophysical mechanism distinct from standard p53 stabilizers.\u003c\/p\u003e\n\u003cul data-path-to-node=\"22\"\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"22,0,0\"\u003e\u003cb data-path-to-node=\"22,0,0\" data-index-in-node=\"0\"\u003eMDM2 Targeting:\u003c\/b\u003e The p53 segment binds to MDM2 proteins located on the surface of cancer cells (but absent on normal cells).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"22,1,0\"\u003e\u003cb data-path-to-node=\"22,1,0\" data-index-in-node=\"0\"\u003ePore Formation:\u003c\/b\u003e Upon binding, the peptide undergoes a conformational change, assembling into oligomers that penetrate the cell membrane.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"22,2,0\"\u003e\u003cb data-path-to-node=\"22,2,0\" data-index-in-node=\"0\"\u003eRapid Lysis:\u003c\/b\u003e These oligomers form transmembrane pores (approx. 37 nm diameter), causing immediate leakage of intracellular contents and necrotic cell death, independent of intracellular p53 status.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-path-to-node=\"23\"\u003e\n\u003ch3 data-path-to-node=\"24\"\u003e\u003cb data-path-to-node=\"24\" data-index-in-node=\"0\"\u003eKey Research Applications\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp data-path-to-node=\"25\"\u003e\u003cb data-path-to-node=\"25\" data-index-in-node=\"0\"\u003e1. Malignant Cell Research\u003c\/b\u003e Investigated for its selective cytotoxicity against pancreatic (MiaPaCa-2), breast (MCF-7), and ovarian (SK-OV-3) cancer lines. Research shows it induces \u003cb data-path-to-node=\"25\" data-index-in-node=\"181\"\u003e100% cell death\u003c\/b\u003e in susceptible lines within 24-48 hours.\u003c\/p\u003e\n\u003cp data-path-to-node=\"26\"\u003e\u003cb data-path-to-node=\"26\" data-index-in-node=\"0\"\u003e2. Non-Apoptotic Pathways\u003c\/b\u003e Used to study cell death mechanisms that bypass traditional apoptosis resistance (e.g., Bcl-2 overexpression), offering a model for treating drug-resistant phenotypes.\u003c\/p\u003e\n\u003cp data-path-to-node=\"27\"\u003e\u003cb data-path-to-node=\"27\" data-index-in-node=\"0\"\u003e3. Stem Cell Selectivity\u003c\/b\u003e Studies indicate PNC-28 targets leukemia stem cells (LSCs) while sparing normal hematopoietic stem cells, making it a valuable probe for selective oncology research.\u003c\/p\u003e\n\u003chr data-path-to-node=\"28\"\u003e\n\u003ch3 data-path-to-node=\"29\"\u003e\u003cb data-path-to-node=\"29\" data-index-in-node=\"0\"\u003eStorage \u0026amp; Handling\u003c\/b\u003e\u003c\/h3\u003e\n\u003cul data-path-to-node=\"30\"\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"30,0,0\"\u003e\u003cb data-path-to-node=\"30,0,0\" data-index-in-node=\"0\"\u003ePhysical State:\u003c\/b\u003e Lyophilized (Freeze-Dried) White Powder.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"30,1,0\"\u003e\u003cb data-path-to-node=\"30,1,0\" data-index-in-node=\"0\"\u003eStorage (Powder):\u003c\/b\u003e Store at -20°C (Freezer). Stable for up to 24 months.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"30,2,0\"\u003e\u003cb data-path-to-node=\"30,2,0\" data-index-in-node=\"0\"\u003eReconstitution:\u003c\/b\u003e\u003c\/p\u003e\n\u003cul data-path-to-node=\"30,2,1\"\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"30,2,1,0,0\"\u003e\u003cb data-path-to-node=\"30,2,1,0,0\" data-index-in-node=\"0\"\u003eSolvent:\u003c\/b\u003e Sterile Water or 10-30% Acetic Acid (if solubility is low).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"30,2,1,1,0\"\u003e\u003cb data-path-to-node=\"30,2,1,1,0\" data-index-in-node=\"0\"\u003eSonication:\u003c\/b\u003e May be required due to the peptide's amphipathic nature.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"30,3,0\"\u003e\u003cb data-path-to-node=\"30,3,0\" data-index-in-node=\"0\"\u003eStorage (Liquid):\u003c\/b\u003e Store at 4°C. Use within 14 days. \u003cb data-path-to-node=\"30,3,0\" data-index-in-node=\"52\"\u003eAvoid freeze-thaw cycles.\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-path-to-node=\"31\"\u003e\n\u003ch3 data-path-to-node=\"32\"\u003e\u003cb data-path-to-node=\"32\" data-index-in-node=\"0\"\u003eDisclaimer\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp data-path-to-node=\"33\"\u003e\u003cb data-path-to-node=\"33\" data-index-in-node=\"0\"\u003eFOR RESEARCH USE ONLY.\u003c\/b\u003e This product is a chemical compound intended strictly for laboratory research and development. It is not approved by the FDA for clinical use, diagnostics, or therapeutic procedures in humans or animals. The physiological and toxicological properties of this chemical have not been fully investigated.\u003c\/p\u003e","brand":"Peptagon","offers":[{"title":"Default Title","offer_id":47712473153750,"sku":"PNC28-20","price":165.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0783\/3085\/5638\/files\/PNC-28-20mg.png?v=1768510757","url":"https:\/\/peptagon.com\/products\/pnc-28-p53-derived-research-peptide","provider":"Peptagon","version":"1.0","type":"link"}